Dear Authors,
If you believe that your paper was mistakenly rejected by other leading journals and you do not agree with final decision, the editors of Reports of Practical Oncology and Radiotherapy offer new fast track review. You may submit your manuscript to Reports of Practical Oncology and Radiotherapy together with all prior peer-reviews obtained from the other journal and your rebuttal letter. We guarantee review based decision within 72 hours from the time we will receive your manuscript.

Fast track submission process: Please submit the manuscript with all reviews and rebuttal letter by email to Dr. Michal Masternak ( for fast review processing. To assure immediate attention the email title must to include: RPOR-fast track- Last Name First Name (of corresponding author).

Volume 16, Number 6, 2011

Bystander effects induced by direct and scattered radiation generated during penetration of medium inside a water phantom

Maria Konopacka, Jacek Rogoliński, Krzysztof Ślosarek


Background: The biological effects of ionizing radiation have long been thought to results
from direct targeting of the nucleus leading to DNA damage. Over the years, a number of
non-targeted or epigenetic effects of radiation exposure have been reported where genetic
damage occurs in cells that are not directly irradiated but respond to signals transmitted
from irradiated cells, a phenomenon termed the “bystander effects”.
Aim: We compared the direct and bystander responses of human A 549, BEAS-2-B and
NHDF cell lines exposed to both photon (6 MV) and electron (22 MeV) radiation inside a
water phantom. The cultures were directly irradiated or exposed to scattered radiation 4 cm
outside the field. In parallel, non-irradiated cells (termed bystander cells) were incubated
in ICM (irradiation conditioned medium) collected from another pool of irradiated cells
(termed donor cells).
Materials and methods: In directly irradiated cells as well as ICM-treated cells, the frequency
of micronuclei and condensation of chromatin characteristic for the apoptotic process were
estimated using the cytokinesis-block micronucleus test.
Results: In all tested cell lines, radiation induced apoptosis and formation of micronuclei.
A549 and BEAS-2B cells cultured in ICM showed increased levels of micronuclei and apoptosis, whereas normal human fibroblasts (NHDF line) were resistant to bystander response. In A549 and BEAS-2B cells placed outside the radiation field and exposed to scattered radiation the formation of micronuclei and induction of apoptosis were similar to that after ICM-treatment.
Conclusion: Results suggest that the genetic damage in cells exposed to scattered radiation is caused by factors released by irradiated cells into the medium rather than by DNA damage
induced directly by X rays. It seems that bystander effectsmay have important clinical implications for health risk after low level radiation exposure of cells lying outside the radiation
field during clinical treatment.

Signature: Rep Pract Oncol Radiother, 2011; 16(6) : 256-261

« back


Indexed in: EMBASE®, the Excerpta Medica database, the Elsevier BIOBASE (Current Awareness in Biological Sciences) and in the Index Copernicus.